Designing data policy and governance for smart cities: theoretical essay using the IAD framework to analyze data-driven policy / Diseño de políticas y gobernanza de datos para ciudades inteligentes: ensayo teórico utilizando el marco IAD para analizar políticas basadas en datos / Desenhando políticas e governança de dados para cidades inteligentes: ensaio teórico com o uso da IAD Framework para analisar políticas orientadas por dados

Abstract This article examines data-driven policy for smart cities and how the institutional analysis and development framework (IAD) is a tool to analyze data governance and data policy design. The research assumes that data-driven policy underpins smart city initiatives and incorporates collective action dilemmas, directly impacting policy design and data governance. The article's motivation is to examine the elements of the IAD framework and decompose the constitutive components of data policy in smart cities, obtaining principles that guide data governance in complex situations of digital transformation. The article is a theoretical essay to discuss data policy in smart cities and how policy design understands dilemmas and conflicts of collective action motivated by data control.

Resumen Este artículo examina la política basada en datos para ciudades inteligentes y cómo el Institutional Analysis and Development Framework (IAD) constituye una herramienta para analizar el diseño de la gobernanza de datos y de la política de datos. El examen realizado en el artículo parte de la premisa de que la política basada en datos, que sustenta las iniciativas de ciudades inteligentes, incorpora dilemas de acción colectiva e impacta directamente en el diseño de las políticas y de la gobernanza de datos. La motivación para la elaboración del artículo fue examinar los elementos del IAD framework y descomponer los componentes constitutivos de la política de datos en las ciudades inteligentes, para luego derivar de ellos los principios que guían la gobernanza de datos en situaciones complejas de transformación digital. El artículo es un ensayo teórico que pretende generar discusiones sobre la política de datos en las ciudades inteligentes y cómo el diseño de políticas entiende los dilemas y conflictos de la acción colectiva motivados por el control de datos.

Resumo Este artigo examina a política orientada por dados para cidades inteligentes e como o Institutional Analysis and Development Framework (IAD) se posiciona como uma ferramenta para analisar o design da governança de dados e de política de dados. A pesquisa realizada neste artigo sobre políticas baseadas em dados parte da premissa de que um projeto orientado por dados sustenta iniciativas de cidades inteligentes e incorpora dilemas de ação coletiva, impactando diretamente o desenho da política e da governança de dados. A motivação do artigo foi examinar dos componentes da estrutura IAD e componentes constitutivos da política de dados que orientam sua governança em sistemas complexos de transformação digital. O artigo é um ensaio que pretende levantar discussões sobre dados em cidades inteligentes e como o desenho de políticas compreende os conflitos de ação coletiva motivados pelo controle de dados.

Leukotriene B4 in equine asthma syndrome: what do we know so far? / Leucotrieno B4 na síndrome asmática equina: o que sabemos até o momento?

The term "equine asthma syndrome" (EAS) was recently proposed due to the resemblance of the equine disease to human asthma. Leukotrienes cause constriction of the bronchi, especially in the lower airways and increase mucus secretion in the respiratory system. Leukotriene B4 (LTB4) has been discovered as a strong chemotactic factor, which plays a role in neutrophil migration. The immunologic background of EAS remains not fully elucidated despite many studies on the pathogenesis. This study aimed to evaluate the LTB4 concentration in the bronchoalveolar lavage fluid (BALF) of horses with and without pulmonary inflammatory disease. Thirty-five mixed breed horses were studied and LTB4 was determined by using specific ELISA Kit. The horses were grouped by 2 different criteria for statistical analysis of data: 1) according to the values for BALF citology and 2) according to the detection of LTB4 in BALF. There was significant difference of effect of age on the LTB4 detection in equine BALF. Younger animals were the majority where it was possible to detect LTB4 values in LBA. In conclusion, there was an effect of age on the detection of LTB4 in equine BALF, where LTB4 levels were more easily detected in younger animals than older animals and the results of this study raise the possibility of considering future studies with the objective of establishing the real role and the best moment to detect LTB4 in BALF of the equine asthma syndrome.(AU)

Recentemente, o termo "síndrome da asma equina" (SAE) foi proposto devido à semelhança da doença equina à asma humana. Os leucotrienos causam constrição dos brônquios, especialmente nas vias aéreas posteriores e aumentam a secreção de muco no sistema respiratório. O leucotrieno B4 (LTB4) foi descoberto como um forte fator quimiotático, que desempenha um papel na migração de neutrófilos. O fundo imunológico do SAE permanece não completamente elucidado apesar de muitos estudos sobre a patogênese. Este estudo teve como objetivo avaliar a concentração de LTB4 no lavado broncoalveolar (LBA) de equinos com e sem doença inflamatória pulmonar. Trinta e cinco cavalos de raças mistas foram estudados e o LTB4 foi determinado usando o kit ELISA específico. Os animais foram agrupados por dois critérios diferentes para análise estatística dos dados: 1) de acordo com os valores para citologia do LBA e 2) de acordo com a detecção do LTB4 no LBA. Houve diferença significativa do efeito da idade na detecção do LTB4 no LBA equino. Os animais mais jovens foram a maioria onde foi possível detectar os valores de LTB4 no LBA. Em conclusão, houve um efeito da idade na detecção de LTB4 em LBA equino, onde os níveis de LTB4 foram mais facilmente detectados em animais jovens do que em animais mais velhos e foi possível detectar a concentração de LTB4 no LBA equino e os resultados deste estudo levantam a possibilidade de considerar futuros estudos com o objetivo de estabelecer o real papel e o melhor momento para detectar LTB4 no LBA da síndrome asmática equina.(AU)

Clinical efficacy of acupuncture and moxibustion in the adjuvant treatment of internet addiction disorder / 中国基层医药

Objective@#To investigate the clinical efficacy and safety of acupuncture and moxibustion in the adjuvant treatment of internet addiction disorder.@*Methods@#From January 2013 to April 2015, a total of 38 patients with internet addiction disorder were randomly selected and divided into study group(19 cases) and control group(19 cases) according to the odd and even order of hospitalize.The control group received psychological therapy, antianxiety therapy and antidepression therapy.The study group received acupuncture and moxibustion in addition to all therapies of the control group.The patients were treated for 8 weeks.Chiese Internet Addiction Scale(CIAS), Family Assessment Device(FAD), HAMD, HAMA were used to determine the internet addiction severity and social function of the two groups at baseline and the 8th weekend of treatment, and TESS at the 8th weekend of treatment.@*Results@#At the 8th weekend of treatment, the scores of CIAS, FAD, HAMA+ HAMD in the two groups were all significantly lower than baseline[(86.8±13.6)points vs.(38.3±11.5)points, t=11.872, (85.6±12.3)points vs.(54.6±18.2)points, t=7.123; (185.8±31.5)points vs.(80.6±29.4)points, t=10.643, (182.7±12.7)points vs.(110.5±32.3)points, t=9.068; (53.4±7.6)points vs.(21.7±9.7)points, t=11.215, (55.2±8.3)points vs.(35.2±8.3)points, t=7.428)](all P<0.05). At the 8th weekend of treatment, the scores of CIAS, FAD, HAMA+ HAMD in the study group were significantly lower than those in the control group(t=3.301, 2.985, 10.825, all P<0.05). There was no statistically significant difference in the incidence rate of side effects between the two groups(all P>0.05).@*Conclusion@#Acupuncture and moxibustion is effective and safe in the treatment of patients with internet addiction disorder.

Construction,expression of I-Ad/IgG2b Fc dimer fusion protein and its identification / 中国免疫学杂志

Objective:To construct I-Ad/IgG2b Fc baculovirus expression vector and express I-Ad/IgG2b Fc dimer fusion protein in Sf9 insect cells.Methods:I-Ad α,I-Ad β and IgG2b Fc gene sequences were amplified from BALB/c mouse lymphocytes by RT-PCR.I-Ad α and I-Ad β were connected with the leucine zipper sequence Fos and Jun respectively by overlapping PCR to form I-Ad α-Fos and I-Ad β-Jun.I-Ad α-Fos and IgG2b Fc fragments were ligated by restriction sites Xba I to form I-Ad α-Fos-IgG2b Fc recombination sequence.I-Ad α-Fos-IgG2b Fc and I-Ad β-Jun fragments were inserted to PPH and PP10,which were the downstream of the promoters in the plasmid pFastBacTMDual,to form pFastBacTMDual+[I-Ad/IgG2b Fc] recombinant plasmids.The constructed vector was identified by PCR,restriction endonuclease and sequencing.The recombinant plasmids pFastBacTMDual+[I-Ad/IgG2b Fc] was transferred into the DH10Bac competent cell to form recombinant baculovirus Bacmid+[I-Ad/IgG2b Fc].The recombinant baculovirus was transfected into Sf9 insect cells by liposome transfection reagent.After infected with Sf9 insect cells,the supernatant was collected and concentrated by PEG20000 to obtain I-Ad/IgG2b Fc dimer fusion protein.The fusion protein was detected by double-antibody sandwich ELISA and Western blot.Results:PCR,restriction enzyme digestion and sequencing confirmed that the recombinant vector pFastBacTMDual+[I-Ad/IgG2b Fc] had the correct sequence.The double antibody sandwich ELISA and Western blot showed that recombinant bacmid could successfully infect Sf9 insect cells,and the expressed fusion protein had the correct conformation.Conclusion:The pFastBacTMDual+[I-Ad/IgG2b Fc] baculovirus expression vector was successfully constructed and expressed in Sf9 insect cells,laying a foundation for the study of I-Ad-restricted T cells.

Comparison of the reliability and validity of two incontinence-associated dermatitis risk assessment instruments in the patients with incontinence / 中国实用护理杂志

Objective To compare the reliability and validity of 2 incontinence- associated dermatitis (IAD) risk assessment tools (IAD risk assessment scale and PAT) in patients with incontinence, and to search for the best risk assessment tool. Methods A total of 101 IAD cases were chosen from the Affiliated Hospital of Nantong University.Reliability was evaluated by the inter- rater reliability,internal consistency,item sensitivity analysis and retest reliability; validity was evaluated by the structure validity and predictive validity. Results Cronbach α was 0.313 (IAD risk assessment scale) and 0.421(PAT);Cronbach α coefficient was the highest after the items that Change sheets and mats and relevant factors were removed respectively in two tools,which were 0.431, 0.428; The correlation between the total scores were 0.711 (P<0.01)(IAD risk assessment scale)and 0.498 (PAT)(P<0.01);Area under curve were 0.661 (IAD risk assessment scale),0.864 (PAT); the best cut-off value to forecast a poor prognosis was 53.5 (IAD risk assessment scale),6.5 (PAT). Conclusions The reliability and validity of IAD risk assessment scale and PAT are low. The change of sheets and mats and Related influencing factors are needed to be adjusted and revised.

Atividade da fosfatase alcalina no lavado broncoalveolar de equinos de policiamento montado no Estado do Rio de Janeiro / Alkaline phosphatase activity in bronchoalveolar lavage of police horses in Rio de Janeiro State, Brazil

A utilidade da determinação das atividades enzimáticas no trato respiratório posterior como ferramenta diagnóstica já foi demonstrada em várias espécies. Nesse contexto, este trabalho teve por objetivo determinar a atividade da Fosfatase Alcalina (FAL) no lavado broncoalveolar (LBA) de equinos da Polícia Militar do Estado do Rio de Janeiro, comparando animais sadios com portadores assintomáticos de doença inflamatória das vias aéreas (DIVA). Para tal, foram avaliados 28 animais adultos, machos, sem histórico de doença respiratória nos dois meses anteriores ao estudo, com os resultados dos exames físicos e laboratoriais (FAL sanguínea, hematócrito, leucograma, proteína total e fibrinogênio plasmáticos) dentro dos parâmetros fisiológicos. Os equinos foram divididos em dois grupos de acordo com o resultado da citologia broncoalveolar. A determinação da atividade da FAL foi realizada por meio de espectrofotometria a partir de alíquotas do sobrenadante do LBA preservadas em nitrogênio líquido. Para a estimativa do fluido epitelial pulmonar e da atividade da FAL neste, foi realizada a correção da diluição provocada pelo lavado. Os equinos com contagem diferencial de tipos celulares compatível com DIVA apresentaram atividade de FAL no LBA menor, quando comparados aos animais sadios, podendo essa dosagem ser utilizada como complementação do diagnóstico da DIVA.

The use of determining the enzymatic activities in the posterior respiratory tract as a diagnostic tool has already been demonstrated in several species. In this context, this paper aims to determine the activity of alkaline phosphatase (ALP) in the bronchoalveolar lavage (BAL) of horses from the Military Police of the State of Rio de Janeiro, comparing healthy animals with asymptomatic carriers of an inflammatory airway disease (IAD). Twenty-eight adult male animals with no history of respiratory diseases in the last two months prior to the study were studied. Physical exam and blood laboratory test results (ALP, hematocrit, leukogram, total protein and plasma fibrinogen) were within physiological parameters. The equines were separated into two groups according to the results of the bronchoalveolar cytology. The determination of ALP was done by spectrophotometry with aliquots of the supernatant of the BAL preserved in liquid nitrogen. To estimate pulmonary epithelial lining fluid and ALP activity, correction of the dilution caused by the lavage was done. The horses with a cell type differential count compatible with IAD presented a lower ALP activity in BAL when compared to healthy animals, therefore this dosage can be used as a complement in the diagnosis of IAD.

Effect of Counseling on Personality Feature and Self-conscious Symptom of College Students with Internet Addiction Disorder / 中华医学教育探索杂志

Objective:To know the effect of counseling on personality feature and self-conscious symptom of college students with internet addiction disorder (IAD).Methods:Before and after counseling,38 students with IAD in Guangdong Medical College (GDMC)were measured by Sixteen Personality Factor Questionnaire (16PF)and Symptom Check List 90 (SCL-90).Results:After counseling,these students with IAD had higher scores of 4 factors in 16PF(sociability,intelligence,perseverance,autonomy),had lowerer scores of 5 factors in 16PF (daring,suspicion,illusion,anxiety,tensity),and had lower scores of 6 factors in SCL-90 (force,interpersonal sensitivity,depression,anxiety,hostility,terror)(P＜0.05 or P＜0.01).Conclusion:To college students with IAD, counseling could enhance their positive personality features,weaken their negative personality feature and mitigate their self- conscious symptom.

The Analysis on the Relational Factors of Pathological Internet Use among the Post-graduate Medical Students in Tianjin / 中国慢性病预防与控制

Objective To explore the epidemic characters of Pathological Internet Usage (PIU) in the newly enrolled post-graduates and further to detect the relationship between PIU and relative social environment, etc., so as to provide more evidence for the etiology of PIU. Methods Questionnaire was conducted in 556 newly enrolled post-graduate students of a medical university. The recovery rate of the valid questionnaires was 94.72%, and the data were analyzed by chi-square test, t-test and logistic regression. Results The rate of internet-utilizing students in recent one year was 95.3%, thereinto, 5.3% of them was with internet addiction disorder. Multivariate logistic regression analysis showed that the major impact factors were gamble, playing games, the heavy study pressure, psychoticism personality characteristic tendency and low self-respect, the OR values (95%CI) were 13.966 (1.662~117.835), 3.440 (1.401~8.450), 1.249 (1.096~1.423), 0.889 (0.821~0.963) and 2.749 (1.167~6.475), respectively (all P

An Analysis of Psychological Risk Factors of the Medical Postgraduates' Internet Addiction / 中华医学教育探索杂志

Objective:To explore the psychological risk factors of internet addiction disorder use(IAD) among the first grade graduate students of medical science,and to provide evidence for the prevention against IAD.Methods:Total 556 students completed a questionnaire including self-designed General Conditions Questionnaire,Symptom Checklist-90(SCL-90),Self-esteem Scale(SES),Eysenck Personality Questionnaire(EPQ) and Chinese Internet Addiction Scale-Revised(CIAS-R).The recovery rate of the valid questionnaires was 94.72%.Logistic multivariable analysis was used to find out the psychological risk factors.Results: One-factor analysis shows that significant related factors that emerged as common correlates with IAD were sex,present live-condition approval,study pressure,the level of mental health condition,personality characteristic,self-esteem etc.Multiple logistic regression analysis showed that the major impact factors were the heaver study pressure,psychiatric personality traits tendency and low self-esteem.Conclusion:the graduates IAD is closely connected with psychological factors.The relevant departments should pay much attention to their mental health,and guide them to use internet reasonably to avoid affecting mental and body health.

Internet Addiction Disorder and Sensation Seeking of Middle School and High School Students / 中国心理卫生杂志

Objective: Investigate the characteristics of Internet Addiction Disorder and Sensation Seeking of middle school and high school students, and the relationship between addiction and sensation seeking. Methods: Internet Addiction Test Questionnaire and Zuckerman's Sensation Seeking Scale were administered to 307 students in a middle school and a high school in Beijing. Results: Sex and grade differences obviously existed in IAD. Boys and middle school students were easier to be addicted to Internet. The score of Boredom Susceptibility of sensation seeking was positively correlated to IAD. Conclusion: Certain relationship between IAD and sensation seeking did exist.

Comprehensive Intervention on Internet Addiction of Middle School Students / 中国心理卫生杂志

Objective: To explore the effect of psychotherapy in treating middle school students with IAD (internet addiction disorder). Methods: 23 cases of middle school students with IAD had been treated by cognitive therapy or behavior therapy. The IAD scale, SCL-90 scale and SDS scale were used to evaluate the effect before and after therapy. Results: After therapy, the total scores and most dimensions scores in IAD scale, SCL-90 scale and SDS scale decreased significantly than that before therapy. Conclusions: Psychotherapy especially cognitive therapy and behavior therapy is an effective in treating middle school students with IAD.

A Study on Health Related and Eating Related Behaviors by Self-Recognized Health Status / 대한지역사회영양학회지

The aim of this study was to investigate health-related and eating-related behaviors as part of self-recognized health status. The survey was conducted among 304 elderly people in Chunchon city in 1999. Fifty two percent(52%) of the respondents recognized they were healthy, 32% felt so-so and 16% thought themselves unhealthy. When they felt healthy, they engaged in more social work and regular exercise, had better appetites, lower conflict scores with their children, lower depression, higher satisfaction in life, better physical condition(eye, ear, tooth, mentality and walking), and higher ability of ADL(activities of daily living) and IAD(instrumental activities of daily living). Also, the self-recognized group consumed each food groups (meats, green, yellow and white vegetables, fruits, milks, seaweeds, beans) more often and showed a higher preference of food. The results of this study indicate that self-recognized health status affects every pattern of life among the elderly. As a result, comprehensive education(such as nutrition, health, physical and psychological education) should be offered to the elderly.

The Influence of Parental Rearing Styles of Medical Students with IAD on Their Mental Health / 中国医学伦理学

Objective: To study whether parental rearing styles of medical students with IAD are related to their mental health, in order to provide foundation for the prevention and control of IAD. Method: Medical students in grade 1 to 4 in Chongqing Medical University were assessed with SCL-90, EMBU, Internet Addiction Disorder Test,and a compare analysis was made for IAD group and non-IAD group. Results: 6.49% subjects can be defined as IAD according to their testing scores of Internet Addiction Disorder Test. The results of EMBU demonstrated that students in IAD group got more punishment, negation and less care or understanding than those in non-IAD group. The results of SCL-90 demonstrated that IAD group got higher scores than non-IAD group. Parental rearing styles of medical students with IAD are related to their mental health. Conclusion: Medical students with IAD got more punishment, negation and less care or understanding,and have lower mental health than those with non-IAD. Negative parental rearing styles of medical students with IAD have bad effects on their development of normal mentality. Parents should pay more attention to their rearing styles.

Research on the Redressing the undergraduates' IAD / 中国医学伦理学

IAD(Internet addiction disorder) has become a new problem in the present situation.Presently,the theoretical research on this problem is abundant while the practice is comparatively deficient.Faced with this problem,we,engaged in students' work,attempt to research into the redressing of the undergraduates' TAD,in order to achieve the goal of moral education.

Construction of pIRES-I-A~d?? and stable expression of BALB/c mouse I-A~d?? chain genes in NIH3T3 cell line / 中国免疫学杂志

Objective:To construct pIRES-I-Ad?? bicistronic eukaryotic expression vector. Methods: Total RNA was acquired by TRIZOL method, the genes of I-Ad ?? chains were amplified through RT-PCR, respectively. The target genes were connected to pGEM-T vector and sequenced. Then the target genes were subcloned into pIRES bicistronic eukaiyotic expression vector and NIH3T3 cell line was transfected. Transfectant was screened by G418 antibiotics. Total RNA of transfectant was obtained by TRIZOL method, mRNA of foreign gene was examined by RT-PCR. Flow cytometry( FCM) was used to detennine foreign gene expression in protein level and surface expression in NIH3T3 cell line. Results: Bicistronic eukaryotic expression vector pIRES-I-AdaB was established. Foreign gene in mRNA level in transfectants was examined. Verified Ⅰ-Ad ?? chain wa3 expressed in high level expressed on transfected NIH3T3 cell line surface by FCM. Conclusion:Bicistronic eukaryotic expression vector pIRES-I-Ad ?? was constructed successfully. It is useful for studying antigen presentation and interaction between epitopes and MHC- Ⅱ molecule of BALB/c mouse.